Nikon E800 Manual de Instruções

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1. Fluorescent Imaging

DSimaging L LC

Note: These instructions were written for the microscope, equipped with a Diagnostic

Instruments SPOT camera, located in the Life Science Microscopy Facility at Purdue

University.

1. The Nikon E800 Microscope.

The Nikon E800 is a fixed-stage upright microscope configured to image in

transmitted light, DIC, and epifluorescence modes. The Nikon is equipped with the

following objectives:

2X/0.1 NA

Plan Apo -------

10x.0.45 NA Plan Apo DIC L

20x/0.75 NA Plan Apo DIC M

40x/0.9 NA

Plan Apo DIC M

100x/1.40 NA Plan Apo DIC H

The last objective (shaded) is an Oil immersion objective.

The condenser is: C-CU with 0.9 N.A. dry lens (swing-out) And contains the

following inserts: A(bright field) /DICL/ DICM/ DICH

DIC lens prisms are inserted above each individual objective lens.

The Nikon also has a filter cube slider with the following filter cubes (right to left):

empty (VIS)

Red (Rhodamine):

11001 Blue (GFP longpass) 470±20/500/515

41017 Green (GFP)

11000 Ultraviolet ( DAPI)

Camera: the microscope is equipped with a SPOT RT slider CCD camera from

Diagnostic Imaging. It can be used as a B/W camera with the color filter sets

removed or as a color camera with the filters inserted.

Nikon E800 Operating Instructions

∞/-

∞/0.17

560±20/585/620

470±20/495/525±25

360±20/400/420

WD = 8.5mm

WD = 4.0mm

WD = 1.0mm

WD = 0.11-0.23mm

WD = 0.13mm

longpass

narrow band

longpass

Oil