Nikon E800 Manual de Instruções - Página 5
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Taking an Image in Brightfield Mode:
1) Complete the Koehler alignment. Find an appropriate area of your sample and focus.
2) Open the SPOT Advanced program.
3) Select Brightfield-grayscale or brightfield-RGB in the lower right of the SPOT screen.
4) You need to do a color balance to show the camera what is white if you are doing color
imaging. The camera program will then assign the other color values.
a. Move to a clear area of your slide.
b. Click on the color balance icon on the menu bar (on the right).
c. Proceed to follow instructions to do the color balance. The values will be saved
in the Brightfield-Color dialogue box.
5) Move back to your sample, focus using the live window, and then capture an image using the
camera icon on the upper right.
6) Select "SETUP > Image Setup" and a dialog box will appear. Click on "Modify". This will
open the main dialogue menu for brightfield
imaging.
a. Select Exposure dialogue box and make
sure "auto" is selected. It should also
be set to:
b. Select "auto". This brings up another
dialogue box.
7) Modify adjustment setting to darken or lighten your image.
Name
Bits
p er
P ixel
Adjustment
Factor
in your image. Lower # gives more contrast.
9) Take another test exposure and repeat above until you have the desired image.
8-bit for grayscale
24-bit for color
no binning
Adjustment: use this to adjust image brightness: lower # is darker image.
Gain: This is usually set at "0" for BF or DIC imaging.
8)
Go to Post-Processing. Modify "Gamma" setting to adjust contrast
5
Color
B alance
s ettings:
based
o n
y our
w hite
balance
c alibration.
Gain:
E lectronic
enhancement
> >noise