Nikon E800 Руководство по эксплуатации - Страница 5

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Taking an Image in Brightfield Mode:

1) Complete the Koehler alignment. Find an appropriate area of your sample and focus.

2) Open the SPOT Advanced program.

3) Select Brightfield-grayscale or brightfield-RGB in the lower right of the SPOT screen.

4) You need to do a color balance to show the camera what is white if you are doing color

imaging. The camera program will then assign the other color values.

a. Move to a clear area of your slide.

b. Click on the color balance icon on the menu bar (on the right).

c. Proceed to follow instructions to do the color balance. The values will be saved

in the Brightfield-Color dialogue box.

5) Move back to your sample, focus using the live window, and then capture an image using the

camera icon on the upper right.

6) Select "SETUP > Image Setup" and a dialog box will appear. Click on "Modify". This will

open the main dialogue menu for brightfield

imaging.

a. Select Exposure dialogue box and make

sure "auto" is selected. It should also

be set to:

b. Select "auto". This brings up another

dialogue box.

7) Modify adjustment setting to darken or lighten your image.

Name

Bits p er P ixel

Adjustment

Factor

in your image. Lower # gives more contrast.

9) Take another test exposure and repeat above until you have the desired image.

8-bit for grayscale

24-bit for color

no binning

Adjustment: use this to adjust image brightness: lower # is darker image.

Gain: This is usually set at "0" for BF or DIC imaging.

Go to Post-Processing. Modify "Gamma" setting to adjust contrast

Color B alance s ettings:

based o n y our w hite

balance c alibration.

Gain: E lectronic

enhancement > >noise