Nikon N-STORM Manual de operação - Página 24
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Adjust the power of the activation laser, if necessary,
during real time analysis (preview) of the acquired
images.
Image acquisition is started. The result of a real time
analysis (preview) and a graph are displayed.
Recommended number of bright points
The recommended data for STORM analysis is as
follows: the density of bright points is not too low but not
so high that they do not overlap each other. For
example, a structure that covers the entire screen (such
as a microtubule in a cell) should be provided with about
100 to 200 bright points per frame in a visual field with
256 x 256 pixels. The number of bright points to be
activated can be adjusted by changing the power of the
activation laser.
Details of real time analysis
During real time analysis, the value of [Minimum Height] on the N-STORM control window is used as a threshold to
analyze bright points. Real time analysis does not include drift correction.
Scale setting
: Maximum, minimum, scale width
(Note that the scale width is
adjusted so that it does not overlap
with numbers in the graph.)
Graph of the number of bright points
(for some latest frames)
Graph of the number of bright points
Chapter 2
STORM Microscopy Operation
Real time analysis (preview) screen during image acquisition
Graphic screen during image acquisition
The number of bright points per frame of
imaging probe
Period number
Automatic scroll on/off
Display width (period count)
setting
Period number
20
Zoom magnification
Molecular position
drawing selection
[Cross]/[Gaussian]
(Gaussian representation)
The thickness of cross
Channel display color
Delete any Gaussian
representation/cross
that has already
been drawn from the
display. (Molecule
position data
remains undeleted.)