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2.3

N-STORM Analysis

Analyze an acquired dataset, perform drift correction, and identify the positions of the fluorescent probe molecules.

Click [Analysis GUI] to display the analysis tool.

Click

[File Open].

A dialog box for selecting a STORM image is displayed.

Select the image file (ND2 file) acquired for STORM

analysis and then click [Open].

Saving open data

In the N-STORM analysis window, it is not possible to

open two or more ND2 files at once. For this reason, if a

new file is opened, the currently open file is closed. If, at

this time, the file contains any information that is not

saved, a dialog box is displayed.

- If the currently open molecule list (STORM image) has

not yet been saved, the dialog box shown on the right

is displayed. To save the molecule list, select [Yes]. If

[No] is selected, the latest molecule list will be

discarded.

- If the STORM parameter(s) in the currently open ND2

file have not yet been saved, the dialog box shown on

the right is displayed. To save the parameter(s) to the

ND2 file, select [Yes]. If [No] is selected, any changes

made to the parameter(s) will be discarded.

Results of past analysis

If STORM analysis has already been performed on the

selected ND2 file such that a corresponding molecule list

file exists, a dialog like that shown on the right is

displayed. To perform a new STORM analysis, select

[No]. (If [Yes] is selected, the saved result of the previous

analysis is read.)

Chapter 2

STORM Microscopy Operation