Clontech Laboratories His60 Ni Superflow Cartridges Manual do utilizador - Página 2
Procurar online ou descarregar pdf Manual do utilizador para Equipamento de laboratório Clontech Laboratories His60 Ni Superflow Cartridges. Clontech Laboratories His60 Ni Superflow Cartridges 15 páginas. Purification of expressed his-tagged proteins from bacterial, yeast, mammalian, and baculovirus-infected cells.
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Table of Contents
I.
Introduction ..................................................................................................................................................................... 3
A. His60 Ni Superflow 1 ml & 5 ml Cartridge Specifications ........................................................................................ 3
His60 Ni Superflow Resin .......................................................................................................................................... 4
II.
List of Components ......................................................................................................................................................... 6
III.
Additional Materials Required .................................................................................................................................... 7
A. Equipment ................................................................................................................................................................... 7
Buffers - Native Conditions ....................................................................................................................................... 7
Buffers - Denaturing Conditions - Guanidine-HCl or Urea....................................................................................... 8
D. Enzymes ...................................................................................................................................................................... 8
Accessories for Automated Purification ..................................................................................................................... 8
Accessories for Syringe-Based Purification ................................................................................................................ 8
G. Optional ....................................................................................................................................................................... 8
IV.
Related Products: Extraction Buffers, Protease Inhibitors, and His-Tag Antibodies ................................................. 9
V. Sample Preparation & Purification ............................................................................................................................... 10
A. PROTOCOL: Extracting Proteins from Cells ........................................................................................................... 10
PROTOCOL: Automated Purification on a Liquid Chromatography System .......................................................... 11
PROTOCOL: Manual Purification Using a Syringe ................................................................................................. 12
D. PROTOCOL: Complete Regeneration of His60 Ni Superflow Cartridges ............................................................... 14
VI.
Troubleshooting Guide ............................................................................................................................................. 15
Table of Tables
Table 1. His60 Ni Superflow Cartridge Specifications ........................................................................................................... 3
Table 2. Reagent Compatibility with His60 Ni Superflow Resin (Based on Literature References) ..................................... 5
Table 3. Troubleshooting Guide for His60 Ni Superflow Cartridges ................................................................................... 15
PT5143-1
031716
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Clontech Laboratories, Inc. A Takara Bio Company
Contact Us
Technical Support
tel: 800.662.2566 (toll-free)
fax: 800.424.1350 (toll-free)
web:
www.clontech.com
e-mail:
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