Clontech Laboratories His60 Ni Superflow Cartridges Manual do utilizador - Página 3
Procurar online ou descarregar pdf Manual do utilizador para Equipamento de laboratório Clontech Laboratories His60 Ni Superflow Cartridges. Clontech Laboratories His60 Ni Superflow Cartridges 15 páginas. Purification of expressed his-tagged proteins from bacterial, yeast, mammalian, and baculovirus-infected cells.
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I.
Introduction
Clontech's His60 Ni Superflow Cartridges are prefilled with 1 ml or 5 ml of His60 Ni Superflow resin and are
ready to use for purification of his-tagged proteins using a syringe, peristaltic pump, or liquid chromatography
systems such as ÄKTA or other FPLC systems.
A. His60 Ni Superflow 1 ml & 5 ml Cartridge Specifications
Table 1. His60 Ni Superflow Cartridge Specifications
Support
Bead diameter
Column dimensions (mm i.d.)
1
Maximum pressure
Typical back pressure
Recommended flow rate
2
Maximum flow rate
pH stability short term (≤2 hr)
pH stability long term (>2 hr)
3
Binding capacity
System compatibility
Cartridge body material
Connectors
The maximum pressure that can be used with the Superflow matrix itself is 10 bar. However, stability of the cartridges is
1
only guaranteed up to 5 bar.
2
High flow rates may lead to reduced recovery of 6xHis-tagged protein.
3
Binding capacity may vary from protein to protein.
4
Adaptors may be necessary.
PT5143-1
031716
1 ml Cartridge
0.7 cm x 2.5 cm
1.0 bar, 0.1 MPa (1 ml/min)
1 ml/min (156 cm/hr)
10 ml/min (1,559 cm/hr)
60 mg of AcGFPuv
Automated chromatography systems (e.g., ÄKTA, FPLC, etc.),
4
Clontech Laboratories, Inc. A Takara Bio Company
Superflow 6 (cross-linked agarose)
60–160 μm
5 bar, 0.5 MPa
2.0 bar, 0.2 MPa (5 ml/min)
40 ml/min (1,193 cm/hr)
2–14
3–14
peristaltic pump or syringe
Polypropylene
1/16" (inlet); 1/16" (outlet)
www.clontech.com
5 ml Cartridge
1.6 cm x 2.5 cm
5 ml/min (149 cm/hr)
300 mg of AcGFPuv
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