Olympus BX51 Руководство по эксплуатации - Страница 8

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Также для Olympus BX51: Руководство по эксплуатации (17 страниц), Руководство по эксплуатации (40 страниц), Руководство по эксплуатации (34 страниц), Руководство по эксплуатации (37 страниц)

FLUORESCENCE (Transmitted light)

NOTE: The mercury bulb is fragile. It needs to be cool for 30 minutes before being turned on again. It needs to

stay on for at least 15 minutes.

1. Switch on the mercury lamp by setting the switch (18) to "I" (ON). The indicator (19) should light up.

The bulb will stabilize in 5 ‐10 minutes.

2. Set the microscope for bright field imaging ('Bright field reflected light' page 2, steps 2 – 12.)

Use the reflected light splitter knob (30) to switch between white light and UV light lamps.

3. Turn the filter wheel (17) to select a filter cube:

position # 1. HQ:RDil (red)

4. When using the filter cubes, turn the light intensity down all the way (3).

5. To avoid photobleaching, close the shutter (20) "●"

6. To improve image contrast and to prevent photobleaching other parts of the specimen, pull out the field iris

diaphragm knob (21) so that the image of the field iris diaphragm just circumscribes the field of view.

7. To adjust the brightness and to improve contrast, pull out the aperture iris diaphragm knob (22), the

diaphragm will be smaller.

8. Examine specimen and take a picture, if needed. (See page 9 for details).

9. When finished:

‐ Slide the shutter knob (20) to "●"

‐ Push the knob on the reflected light splitter (30) in all the way.

‐ If mercury bulb has burned for at least 15 minutes, turn it off by setting the main switch (18) to "O".

‐ Lower the stage by turning the focus knob (10) and remove the specimen.

‐ Turn the nosepiece (12) back until the 10x objective is into place.

‐ Push the field iris diaphragm knob (21) and the aperture iris diaphragm knob (22) all the way in.

‐ Turn the filter wheel (17) to BF (bright field).

‐ Return any slide filters (26) (31) (32) to their disengaged positions.

2. DAPI (340‐380 Ex; 400 dichroic; 435‐485 Em)

3. DIC

4. Bright field (BF)

5. Dark field

6. Qdot hxCM (red/blue)

(CLOSED).

(CLOSED) when not acquiring an image.