Olympus IX70 Kullanım Kılavuzu - Sayfa 25
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Screen after hitting "NEW"
Shortcuts to common tasks are located in the
default menu bar on the Left side. Main menus are in
the top toolbar (all options vs common options). If you
are making a new protocol, you need to define what to
collect by using the
(or Cfg Acq button on sidemenu).
Pick wavelength to config, select an "illumination" from
list, pick an exposure and binning; repeat as needed. Note the
"allow items to differ" checkbox—it enables different
gains/exposures for each channel. Do use binning to decrease
image size and increase sensitivity (a must for live cells). 2x2
binning increases sensitivity of the camera 4X, 4x4 increases it
16X. I recommend 4x4 and use either all or at least the ND3
neutral density filter to limit light damage to cells (you should get
thousands of intensity values this way). Running gain at 30X works
without penalty for this camera (default is 1X).
If you need a custom "illumination", there is a menu
under the main CONFIGURE menu. Please create new names for
custom setting vs alter the defaults. You can also save them in your
own directories to avoid other users messing with them.
Configure\ConfigureAcquisition
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