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Screen after hitting "NEW"

Shortcuts to common tasks are located in the

default menu bar on the Left side. Main menus are in

the top toolbar (all options vs common options). If you

are making a new protocol, you need to define what to

collect by using the

(or Cfg Acq button on sidemenu).

Pick wavelength to config, select an "illumination" from

list, pick an exposure and binning; repeat as needed. Note the

"allow items to differ" checkbox—it enables different

gains/exposures for each channel. Do use binning to decrease

image size and increase sensitivity (a must for live cells). 2x2

binning increases sensitivity of the camera 4X, 4x4 increases it

16X. I recommend 4x4 and use either all or at least the ND3

neutral density filter to limit light damage to cells (you should get

thousands of intensity values this way). Running gain at 30X works

without penalty for this camera (default is 1X).

If you need a custom "illumination", there is a menu

under the main CONFIGURE menu. Please create new names for

custom setting vs alter the defaults. You can also save them in your

own directories to avoid other users messing with them.

Configure\ConfigureAcquisition