Olympus IX70 Panduan Pengoperasian - Halaman 3

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The IX70 microscope is a manually operated microscope with automated digital image capture. The

objectives, emission filter cubes, DIC prisms, and focus are all controlled manually. Image capture and

excitation filters are controlled through the software.

Brightfield button and

intensity slider (on front left)

To change the magnification, first lower the objectives by focusing the turret all the way down

with the focus knob (so as not to disturb the sample or bash the objectives into the stage). Then turn the

turret from the base. The objectives are arranged clockwise in order of increasing magnification. Be

aware that when you change the objective MetaMorph will not register the change unless you choose the

magnification from the taskbar as well, making scalebars and measurements incorrect.

We now have more objectives than positions in the nosepiece. Objectives listed below as

Available are not normally kept on the microscope but are available for use, just ask the staff to put it on

for you. DO NOT try to change objectives yourself. We must enforce the China Shop Rule of "you broke

it, you bought it"... Dropping a lens is very bad. NA refers to Numerical Aperture, the resolving ability of

the lens; bigger NA = higher resolution.

Available 2x/NA0.04 dry. This lens yields a field of view over 5mm and yields useful

fluorescence images for very large preps. The brightfield condenser will require defocus (upwards) to

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System Anatomy

DIC shear

adjustment screw

Filter turret

Viewport knob

Focus knob

Fig. 1 Controls on microscope base

Objective turret

Brightfield power switch

(on back of base)