Olympus IX70 Panduan Pengoperasian

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Olympus IX70 Panduan Pengoperasian
UC BSD Light Microscopy Core Facility
Olympus IX70 Multi-parameter Fluorescence Microscope
The IX70 microscope is a manual stand with the ability to collect multi-color fluorescence, differential
interference contrast (DIC) and total internal reflection fluorescence (TIRF), as well as provide optical
sectioning (z-axis stepping) and time lapse imaging. It is equipped with a high sensitivity, high resolution
chilled CCD camera, a green/red or blue/red emission beam splitter for simultaneous two color capture
and MetaMorph + MetaFluor software to control the system.
Examples of types of images that can be captured range from simple image capture (bright field or
Fluorescence), sequential capture of up to 4 fluorescent probes, time lapse imaging, (relatively) high speed
imaging, CFP-YFP FRET studies, ratiometric indicator dyes such the calcium probe Fura-2, etc.
Software provides a wide range of image capture and image processing capabilities. MetaFluor is
optimized for real-time (ratio) image capture with real time region of interest (ROI) intensity/
ratio/calibrated data display. MetaMorph is a more general image capture, processing, morphometry
package. Both packages allow images to be automatically collected and annotated.
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Operations Manual (version 1.0 February '09)
Vytas Bindokas, Director