Olympus IX70 Panduan Pengoperasian - Halaman 31

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Filter cube specs:

DAPI (quad)

DAPI (oly MNU2)

CFP

FITC (wide)

FITC(narrow)

YFP

Reds (quad)

Reds (oly MNG2)

Cy5 (quad narrow)

Cy5 (wide)

Fura-2

Cameleons 2

DIC

Stage heater and perfusion.

We have a homemade heater block that accommodates a standard 35mm dish. The heavy aluminum shell maintains

the dish at set point with constant circulation of water from the bath. This is normally set to 37°C or as high as 39°C to account

for thermal loss between the bath and stage. Use a digital thermometer to check the actual dish temperature, if critical. Heating

the dish will increase the rate of evaporation, a headache for long timelapse studies. If you are doing DIC imaging, you need to

avoid plastic since it degrades the DIC effect. One solution is to use a dish that is fitted with a coverslip as the bottom. You will

need to seal the top for long experiments and you may then need a cover also fitted with glass windows. Moisture will

condense over time, so a double pane design may work better. Another possibility is to perfuse the dish using a pump or

gravity-feed system. It is critical that you consult us on how to set this up so as to avoid flooding the system. If you have a

spill, let us know right away so that it can be cleaned up before damage to the microscope can occur. Do not think you can still

finish the experiment—it's finished! We need to track down leaks quickly since the salts will seep in and corrode/ruin

components. You can use a J-tube to maintain bath levels and to help safeguard against overflow leaks.

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APPENDIX

exciter

380

360-370

436/20

480/40HQ

500/20

530-550

640/20HQ

620/60HQ

340; 380

440/20

dichroic

emission

Quad

450/65

400

420LP

455

480/40

505

510LP

quad

535/30HQ

515

535/30HQ

Quad

630/60

570

590LP

Quad

682/22/75HQ

660

700/75HQ

400

510/40

455

535/35; 485/40

analyzer (polarizer)