Olympus IX70 Panduan Pengoperasian - Halaman 9

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Troubleshooting Guide:

•

No Image by eye, no illumination of

open a filter setting (verify by looking at slide), check to see that you have turned on the mercury arc

power (be sure to power down all electronics before firing it, if this is the case!); 2) check that the

Sutter controller is turned on (turn on); 3) check if the fluorescence/TIRF plunger (behind scope, see

picture on pg. 11) is pulled UP to the fluorescence position. 4) Are too many ND filter sliders pushed

in? Try with only the 25% ND slider in, Fig. 4. 5) Is filter turret (manual) shutter closed? Pull toward

you to open. 6) Is the filter cube seated properly? Try turning the manual filter turret wheel and see if

it settles in. 7) Is there an objective lens in the position you are using?

•

Ugly image, but looked OK by eye?

parts and squint at brights). You can also set display color here. Camera data is 12-bits (4095

brightness values), the computer display is 8 bits per color (255 levels), our eyes see less than 100

shades per color. So you have much ability to manipulate displays. You want to use the same settings

for controls vs. treatments. Also, while

MM will correctly restore any settings you

save to images, most other software will

not use this custom information. (Use the

Edit/Duplicate/AsDisplayed menu to

create an exact likeness for other

applications). 2) check the display

scaling range (two ways to do this). While

it's useful to use autoscaling to find focus,

it won't actually give you an idea of how

bright the data actually is (for this, see the

live histogram along the side of each

image). Using big values to control

(autoscale) display mapping can make the

image really look wrong! (use 0.0 or 0.1,

0.1). Using a fixed range (0 to 4095 is full

range) works until the sample gets too

dim. The Display/ScaleImage menu gives

you display mapping range control too,

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slide. If there is no illumination when using the software to

1) Check the Display/AdjustDigitalContrast menu to see if the

last user has left some strange setting.

Note the RESET button—press it to un-

do any custom display values. You can

check/uncheck the "use display settings

for activated images" box to control

display of data. This tool only affects

the appearance of the data, NOT the

data itself. Images will not save with

these settings. You can use the

GAMMA slider to (nonlinearly)

emphasize the bright or dimmer parts of

the image. Using a gamma=1.3 or so

tends to make the data look more like

what our eyes see (we favor less bright