Olympus IX70 Panduan Pengoperasian - Halaman 9
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Troubleshooting Guide:
•
No Image by eye, no illumination of
open a filter setting (verify by looking at slide), check to see that you have turned on the mercury arc
power (be sure to power down all electronics before firing it, if this is the case!); 2) check that the
Sutter controller is turned on (turn on); 3) check if the fluorescence/TIRF plunger (behind scope, see
picture on pg. 11) is pulled UP to the fluorescence position. 4) Are too many ND filter sliders pushed
in? Try with only the 25% ND slider in, Fig. 4. 5) Is filter turret (manual) shutter closed? Pull toward
you to open. 6) Is the filter cube seated properly? Try turning the manual filter turret wheel and see if
it settles in. 7) Is there an objective lens in the position you are using?
•
Ugly image, but looked OK by eye?
parts and squint at brights). You can also set display color here. Camera data is 12-bits (4095
brightness values), the computer display is 8 bits per color (255 levels), our eyes see less than 100
shades per color. So you have much ability to manipulate displays. You want to use the same settings
for controls vs. treatments. Also, while
MM will correctly restore any settings you
save to images, most other software will
not use this custom information. (Use the
Edit/Duplicate/AsDisplayed menu to
create an exact likeness for other
applications). 2) check the display
scaling range (two ways to do this). While
it's useful to use autoscaling to find focus,
it won't actually give you an idea of how
bright the data actually is (for this, see the
live histogram along the side of each
image). Using big values to control
(autoscale) display mapping can make the
image really look wrong! (use 0.0 or 0.1,
0.1). Using a fixed range (0 to 4095 is full
range) works until the sample gets too
dim. The Display/ScaleImage menu gives
you display mapping range control too,
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slide. If there is no illumination when using the software to
1) Check the Display/AdjustDigitalContrast menu to see if the
last user has left some strange setting.
Note the RESET button—press it to un-
do any custom display values. You can
check/uncheck the "use display settings
for activated images" box to control
display of data. This tool only affects
the appearance of the data, NOT the
data itself. Images will not save with
these settings. You can use the
GAMMA slider to (nonlinearly)
emphasize the bright or dimmer parts of
the image. Using a gamma=1.3 or so
tends to make the data look more like
what our eyes see (we favor less bright
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