Olympus IX70 Panduan Pengoperasian - Halaman 4

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produce illumination over the entire field of view. Be careful when trying to focus this lens on materials

high above the stage—you may cause the high power objectives to push up on the stage plate/specimen

and risk damage to optics. Ask for advice on how to use this lens.

Available 4x/0.16 dry.

10x/0.3 dry

20x/0.5 dry. NOTE this lens has 3 correction caps available to improve images through #1

coverslips, 1-mm thick glass dishes (or a glass slide), and also for plastic (1.1-mm thick). Caps are

unscrewed and replaced with the desired cap. Be sure to place the cap in a plastic holder; holders are

located on the shelf to the right of the scope). Note this lens has poor performance in UV (lousy DAPI

signal).

40x/0.6 LWD dry objective. This has a correction collar for glass/plastic and thickness (0-2 mm).

(not on scope routinely; place into 40x oil position when needed).

Available 40x/1.35 oil UV-optimized. This is a very high resolution objective the best for use

with UV dyes, especially the calcium dye, fura-2. Moderate magnification and high NA make this an

especially bright objective.

60x/1.45 oil, TIRF. The very high NA produces the highest resolution under regular epi

fluorescence. This objective is also optimized to permit the specialized illumination method called Total

Internal Reflection Fluorescence (TIRF; AKA, evanescent wave). TIRF uses laser light to illuminate only

the footprint of a cell attached to a glass coverslip (i.e., the bottom 40 to 200 nm), showing nothing of the

portions above that zone. The high NA yields optical sectioning that approaches that on confocal

microscopes.

Available 60x/1.2 water. Use a hanging drop of water, not oil, on this objective. It provides a

greater working distance than the 60-oil, and when viewing thick preparations it minimizes depth-related

optical distortions.

100x/1.3 oil. Not as bright as either the 46x or 60x oil objectives, but still a nice high mag

objective.

Available 150x/1.4 oil, TIRF The Rolls Royce of objectives. This is a high resolution, high mag

objective that can also be used in TIRF applications. You will need to manually calibrate pixel sizes

when you use this lens.

Brightfield and Fluorescence modes

There are four positions for fluorescence filter cubes in the

manual turret below the objectives. The filter position is

changed by rotating the wheel under the objective turret (see

Fig. 2, "Filter change wheel"). We have four cubes

permanently stationed in the turret: DAPI, FITC long pass, the

Quad mirror and Cy3. The DAPI, FITC long pass and Cy3

cubes will work for both the camera and the oculars (they

have both excitation and emission filters built in) but the

Quad mirror works for the camera only!! The Quad mirror

(ID tag= "4") is for collecting multiple color channels with the

Acquire multiple wavelengths menu in MetaMorph. It will

collect DAPI, FITC (narrow), TexRed and Cy5 channels only

when the appropriate filter button in the taskbar is pushed.

The cube in the light path is indicated by the

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colored

tab,

Fig. 2 right side of scope

Manual

shutter

Filter

wheel

Mag

changer

Filter list