Active Motif 53130 Посібник користувача - Сторінка 14

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Problem/question

Instrument will not

turn on

Sonication run will not

start

Samples do not sonicate

as expected

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Possible cause

Recommendation

No power is being sup-

Check that the power cord is securely plugged into both the

plied to the unit

PIXUL instrument and wall plug.

Unit hasn't been

Ensure that both the power switch on the back side and the

properly turned on

main power switch on the front side of the PIXUL instrument

are both turned on.

Coupling Fluid is not

Check to make sure that the Coupling Fluid has started circulat-

circulating

ing

Plate is installed incor-

Ensure well A1 of the plate is placed in the upper left corner

rectly

Samples are overheat-

Monitor the Coupling Fluid temperature and ensure that it

ing

does not exceed 35°C during sonication. Reduce the Pulse and/

or PRF values if necessary.

Samples are not

Ensure that your sample is diluted in the recommended sonica-

diluted in appropriate

tion buffer, as specified in Quick Guides and User Manuals.

sonication buffer

Consider running a positive control sample, available from

Active Motif.