Olympus CX43-RFAB Інструкція з експлуатації - Сторінка 11

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Olympus CX43-RFAB Інструкція з експлуатації
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Reflected fluorescence observation method

For reflected fluorescence observation, the B excitation fluorescence illuminator is necessary.
(For attaching procedures, see "4 Assembly" on page 10.)
If the analyzer for simple polarization and the polarizer are engaged in the light path, remove them from the light path.
1
Set the main switch of the microscope frame
to (ON).
2
Select BF (brightfield observation).
3
Place the specimen on the stage.
4
Engage the 10X objective in the light path.
5
Bring the specimen into focus and
adjust the brightness.
6
Adjust the interpupillary
7
Adjust the diopter.
8
Engage the objective for phase contrast
in the light path.
9
Engage the objective you use in the light path.
distance.
a
Bring the specimen into focus and
adjust the brightness.
b
Engage the B excitation fluorescence
illuminator in the light path.
c
Select FL (fluorescence observation).
d
e
Set the main switch of the B excitation
fluorescence illuminator to  (ON).
f
Bring the specimen into focus and
adjust the brightness.
Observation
CX43-RFAB
Remove the top lens
of the condenser
from the light path.
7