Nikon N-STORM Посібник з експлуатації - Сторінка 31

Переглянути онлайн або завантажити pdf Посібник з експлуатації для Мікроскоп Nikon N-STORM. Nikon N-STORM 48 сторінок. Super resolution microscope
Також для Nikon N-STORM: Посібник користувача (11 сторінок)

Друкувати сторінку

(4)

Click [OK].

The molecules that exceed the threshold are

removed from their channel, and a new channel in

which they are to be stored is created. The channel

is named [destination-channel-name-Xt]. ("Xt"

stands for crosstalk.)

(5)

Check the details of the

[destination-channel-name-Xt] channel tab. If there

is no problem in merging with the destination

channel, right-click the tab and then select [Merge].

To cancel the destination, right-click the

[destination-channel-name-Xt] channel tab and then

select [Undo]. The state existing before crosstalk

subtraction is restored.

(6)

If STORM images are acquired on two channels,

subtract crosstalk for the other channel in the same

way, by following steps (1) to (5).

In this case, perform the steps up to (5) for the first

channel, and then return to (1) and perform the

same processing for the second channel.

Click

[File Save] to save the STORM parameters.

The parameters for STORM analysis are saved to the ND2

file.

Type of file used by N-STORM

The file formats used by the N-STORM software are as follows.

File type

ND2 file

Result of STORM

analysis in binary

format

Result of STORM

analysis in text

format

Drift correction data

Open the STORM image on the main screen of NIS-Elements

The currently displayed image can be captured and opened on the main screen of NIS-Elements by clicking

[Create New Elements Document from Current View].

Set ROI and zoom in on it

ROI can be set by clicking

according to the size of the window.

Chapter 2

STORM Microscopy Operation

File name

(ND2-file-name).nd2

(ND2-file-name)_list.bin

(Any-file-name).txt

(ND2-file-name)_drift.txt

[Use ROI]. Right-clicking within the specified ROI range displays the ROI range

6-(5)

Set of conventional images acquired through

NIS-Elements. Parameters for STORM analysis (e.g.,

Identification Settings, Display Options, Filter Settings)

are saved into the ND2 file by selecting the ND2 format

in the [File Save] dialog box of the N-STORM analysis

window.

STORM image (molecule list) created through analysis.

This is automatically created by performing N-STORM

analysis. It can also be saved by using the [File Save]

dialog box in the N-STORM analysis window.

A molecule list can be exported in text format by

selecting text format in the [File Save] dialog box of the

N-STORM analysis window. This is used to share the

results of analysis with other software.

This file is automatically created by performing drift

correction. It is internally used by the N-STORM

software.

Description