Consort EVS1100 Manual - Halaman 7

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Gel preparation

This table shows the volume of agarose solution required to make the desired agarose gel (5 mm

thick) for each unit tray size. For a standard 0.7 % agarose gel, add 0.7 g of agarose to 100 ml of 1x

TAE or TBE solution. The same 1x solution should be used in the tank buffer solution.

Add the agarose powder to a conical flask.

Add the appropriate amount of 1 x TAE or TBE solution from the table above. To prevent evaporation

during the dissolving steps below, the conical flask should be covered with parafilm.

Dissolve the agarose powder by heating the agarose either on a magnetic hot plate with stirring bar

or in a microwave oven. If using the microwave method, the microwave should be set at around a

400 W or medium setting and the flask swirled every minute. The solution should be heated until all

crystals are dissolved. This is best viewed against a light background. Crystals appear as translucent

crystals. These will interfere with sample migration if not completely dissolved.

The gel must be cooled to between 50°C and 60°C degrees before pouring.

Gel pouring with model EHS1050

Fit the casting gates into the grooves in the unit which are 10 mm from the platinum wire. Ensure

that these are seated as deeply as possible. This will ensure that a good seal is formed and that

there is no possibility of gel leakage. A small amount of vaseline or sealing grease on the bottom and

side edges of the gates will give further leakage protection.

Place the comb(s) in the grooves. Each tray has more than one comb grove so that multiple combs

can be used. Using multiple combs increases sample number available per gel but decreases run

length and care must be taken to ensure that samples from the first wells do not migrate into the

lanes of the second comb wells.

Pour in the agarose carefully so as not to generate bubbles. Any bubbles that do occur can be

smoothed to the edge of the gel and dispersed using a pipette tip.

Allow the agarose to set, ensuring that the gel remains undisturbed.

Carefully remove the gel casting gates and comb and transfer the gel including tray to the main tank.

Model

Gel Width (cm)

EHS1050

EVS1100

EHS1200

EHS1300

EHS1400

EHS1500

Horizontal Gel Electrophoresis units

Length (cm)

Gel Volume (ml)

113

100

200

250

308

312

416