Consort EVS1100 Manual - Halaman 8
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Gel pouring with casting dams
Fit the casting dams over each end of the tray and place onto a level surface. The dams should be
fitted so that there is no gap between the sides of the tray and the groove in the dams. This will
ensure that there is no possibility of gel leakage.
Place the comb(s) in the grooves. Each tray has more than one comb grove so that multiple combs
can be used. Using multiple combs increases sample number available per gel but decreases run
length and care must be taken to ensure that samples from the first wells do not migrate into the
lanes of the second comb wells.
Pour in the agarose carefully so as not to generate bubbles. Any bubbles that do occur can be
smoothed to the edge of the gel and dispersed using a pipette tip.
Allow the agarose to set, ensuring that the gel remains undisturbed.
Carefully remove the gel casting gates and comb and transfer the gel including tray to the main tank.
Gel pouring using the flexicaster
Level the flexicaster base by adjusting the feet so that the bubble is exactly central.
Insert the desired length tray into the flexicaster such that one end of the tray is pushed up and seals
against the silicone mat of the permanent end of the flexicaster.
Position the movable end of the flexicaster so that the silicone mat is pushed against the other end of
the tray.
Turn the cam so that the silicone mat tightly seals against the side of the tray. Pour in the agarose
carefully so as not to generate bubbles. Any bubbles that do occur can be smoothed to the edge of
the gel and dispersed using a pipette tip.
Allow the agarose to set, ensuring that the gel remains undisturbed.
Carefully remove the gel casting gates and comb and transfer the gel including tray to the main tank.
Horizontal Gel Electrophoresis units
4