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3-6 Immersion Objectives

Fig. 22

Using the Immersion Objectives

#Be sure to use the provided Olympus immersion oil.

1. Focus on the specimen by switching the objectives fro the lowest

power to highest power.

2. Before engaging the immersion objective in the light path, place a

drop of immersion oil provided with the 100X objective combination

model onto the specimen at the area to be observed.

3. Turn the revolving nosepiece to engage the immersion objective, then

focus using the fine adjustment knob.

#Since air bubbles in the oil will affect the image quality, make sure

that the oil is free of bubbles.

a. To check for bubbles, remove the eyepieces and fully open the field

and aperture iris diaphragms, then look at the exit pupil of the

objective inside the observation tube. (The pupil should appear round

and bright.)

b. To remove bubbles, turn the revolving nosepiece to move the oil

immersion objective back and forth a few times.

}If the condenser engraving shows a numerical aperture (NA) of 1.0 or

higher, the number applies only when oil is applied between the slide

glass and the top surface of the condenser. When oil is not present,

the NA is about 0.9.

4. After use, remove oil from the objective front lens by wiping with gauze

slightly moistened with an ether (70%)/alcohol (30%) mixture.

Caution in use of the immersion oil

If immersion oil penetrates in your eye or attaches to your skin,

apply the following treatment immediately.

Eye: Rinse with fresh water (for more than 15 minutes)

Skin: Wash with water and soap.

When the appearance of your eye or skin is altered or pain

continues, immediately consult the doctor.

(Fig. 22)