Olympus IX70 작동 지침 - 페이지 2

{카테고리_이름} Olympus IX70에 대한 작동 지침을 온라인으로 검색하거나 PDF를 다운로드하세요. Olympus IX70 5 페이지. Inverted fluorescent microscope
Olympus IX70에 대해서도 마찬가지입니다: 운영 매뉴얼 (32 페이지)

페이지 인쇄

Fluorescence Imaging Instructions:

1. Turn off transmitted light.

2. Rotate the filter wheel to your desired filter for your specific sample. (Green for

CY3, TRITC; blue of Alexa488 , FITC; UV for DAPI, etc.) (See Fig. 2 for filter

wheel)

a. You should see a colored light coming through the objective lens (i.e. blue,

green)

3. Find an area of your specimen that you want to image using the XY stage control

and fine focus knob.

Imaging Instructions:

1. Pull out slider to block the light to the objective lens (right side level with the

oculars)

a. Third stop, camera icon

2. Open the software on the computer: cellSens Entry

3. Click the live button to turn on the camera

4. Adjust the exposure by moving the slider to right until your image appears

properly exposed.

The exposure normally can be set as auto in bright field image mode and as

manual in fluorescent image mode.

5. Normalize background:

a. For Bright field images: on the top right toolbar, click the white dropper icon

b. For fluorescent images: on the top right toolbar, click the black dropper icon

c. Then select an area of 'background ' on your image slide by drawing a small

box encompassing only the background. This sets the write or black balance for

your image and reduces background noise.

Focus your image to the camera using the fine focus if needed.

Scale Bar:

a. Select scale bar by going to view drop down on the top menu and click on

scal bar tab.

b. Make sure you have the correct value for the object lens

8. Click the Snapshot icon to capture image (next to the live button)

a. Save your image under the user folder on the desktop (if you have scale bar

on your images. When you save images as .tiff file, the images will have two

layers; when you save your images as .jpeg file, the images will only have one

layer)

9. When finished, close the software

10. Turn off microscope and mercury lamp.

··