BD FACSAria Startup, Qc & Shutdown Procedure - Page 5
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1
Inspect sheath filter for air bubbles.
4
If there are any bubbles, bleed the filter after fluidics startup while the tank is pressurized. Be
cautious, as the tank is at high pressure and the bleed valve will need to be slightly loosen to
ensure it does not come off.
1
Note: Remaining air in the sheath filter can cause
instability in the stream during setup and through
your run.
1
Start the stream.
5
Ensure the correct nozzle size is shown in stream window. Start the stream by clicking the red "X"
at the top of the stream window.
Note: If incorrect nozzle size shown
Check the stream image and droplet formation.
6
Confirm with images below to ensure that the droplet formation is normal.
Note: When adjusting the settings
for the droplet formation, never
change the frequency or the gap
that is preset in Diva. These values
are optimized for each nozzle on
each instrument. Only the
amplitude and the drop 1 should
need minor modifications to obtain
optimal droplet formation.
1
Turn on the Sweet Spot.
7
Click the button at the top of the Stream window
off
Bleeder
valve
Sheath filter with
observed air bubble.
FACSAria™ Nozzle Swap SOP for further instructions.
See
on
https://www.bdbiosciences.com/documents/BD_FACSAria_II_User_Guide.pdf