BD FACSAria Startup, Qc & Shutdown Procedure - Page 7
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Optimize Accudrop laser diode.
2
Minimal adjustments should be needed on the laser diode adjustment stage to get the brightest
illumination of the streams.
Check Fanning.
3
Adjust 2
, 3
and 4
nd
rd
Note: Fanning can cause sort impurity.
nd
2
drop is not optimal. Fanning of center stream seen.
Open Accudrop Experiment in QC Folder
4
Select the tube under the specimen and open the Drop Delay Sort Layout under the Global Sheet.
drop in order to minimize any fanning.
th
nd
2
drop optimized. Tight center stream
See FACSAria™ Drop-Charge Delay SOP