Olympus BX51 Operating Manual - Page 11

Browse online or download pdf Operating Manual for Microscope Olympus BX51. Olympus BX51 17 pages. System
Also for Olympus BX51: Instructions Manual (40 pages), Instructions Manual (34 pages), Instructions Manual (37 pages), Operating Manual (11 pages)

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SIMULTANEOUS FLUORESCENCE AND PHASE CONTRAST

1. Remove the microscope cover.

2. To turn on the mercury bulb, set the main switch (18) on the Power Supply Unit to "I"(ON).

The light indicator (19) on the Power Supply Unit should go ON. Wait 5 to 10 minutes for

the mercury bulb to stabilize. The mercury bulb should stay on for at least 15 minutes before

turning off.

3. Setup the microscope for Phase Contrast imaging (see instructions on 'Phase contrast' page

5, #2-26).

4. Engage the fluorescence mirror unit (17) until the proper filter cube is in place:

5. To allow light from the mercury bulb to reach the specimen, slide the shutter knob (20) to

position marked ™.

6. Adjust the transmitted light for the best balance of fluorescence and phase contrast brightness

using the brightness adjustment knob (3).

7. To improve image contrast and to prevent color fading of fluorescent light in other part than

observed region, pull out the field iris diaphragm knob (21) so that the image of the field iris

diaphragm just circumscribes the field of view.

8. To help adjusting the brightness of you the observed image and to improve the contrast, pull

out the aperture iris diaphragm knob (22), the aperture iris diaphragm will get smaller.

9. Examine specimen and document image if necessary:

10. To avoid bleaching, slide the shutter knob (20) to the position marked ˜ whenever you are

not observing the specimen.

11. When finished:

This Manual: http://www.manuallib.com/file/2605418

WU – #1, dichroic mirror DM400, excitation filter BP330-385, barrier filter BA420

WB – #2, dichroic mirror DM500, excitation filter BP450-480, barrier filter BA515

WG – #3, dichroic mirror DM570, excitation filter BP510-550, barrier filter BA590

If using 35mm film, use checklist for 'Olympus 35mm Camera'.

To obtain still digitized images, use checklist for 'Polaroid Digital Microscope

Camera (DMC).

To obtain still digitized images and digitized movies, use checklist for 'Sony, color

Video camera'.

Slide the shutter knob (20) to the position marked ˜.

If mercury bulb has burned for at least 15 minutes, turn off the mercury bulb by

setting the main switch (18) on the Power Supply Unit to "O"(OFF). The light

indicator (19) on the Power Supply Unit should go out.

NOTE: The mercury bulb needs to cool down for at least 15 minutes before turning

back on.

Lower the stage by turning the focus knob (10) towards you and remove the slide

with your specimen from the stage (7).

Turn the nosepiece (12) back until the 10x objective for brightfield imaging is into

place.

Push the field iris diaphragm knob (21) and the aperture iris diaphragm knob (22) al

the way back into the microscope.

Engage the fluorescence mirror unit (17) back to BF (brightfield) - #6

Rotate the condenser turret (23) until the BF (brightfield) mark is visible.

Turn the light intensity down using the brightness adjustment knob (3).