Olympus BX51 Operating Manual - Page 3

Browse online or download pdf Operating Manual for Microscope Olympus BX51. Olympus BX51 17 pages. System
Also for Olympus BX51: Instructions Manual (40 pages), Instructions Manual (34 pages), Instructions Manual (37 pages), Operating Manual (11 pages)

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BRIGHTFIELD

1. Remove the microscope cover.

2. Turn on the halogen light switch (1) for transmitted light to "I" (ON).

3. Check the light path. The light path selector knob (2) should be pushed in all the way.

4. Adjust the light intensity using the brightness adjustment knob (3). The numerals to the right

of the lamp voltage indicator LEDs (4) indicate the voltage.

5. Adjust the interpupillar distance. While looking through the eyepieces, adjust oculars (5)

until the left and right fields of view coincide completely.

6. Make sure the 10x objective (6) is in place.

7. Place the slide with your specimen on the stage (7) and hold it with the specimen holder (8).

8. Find your specimen using the stage controls (9).

9. Focus specimen using fine/course focussing knobs (10).

10. Adjust the diopter:

11. If desired switch to the next objective by rotating the nosepiece (12) and focus. Continue

until you reach the desired magnification.

12. Establish Koehler illumination:

This Manual: http://www.manuallib.com/file/2605418

Operating Manual for the Olympus BX51

Close your left eye and focus on the specimen using the fine focus knob.

Close your right eye and focus on the specimen using the diopter ring (11) on the left

ocular.

Open both eyes and confirm that the focus is comfortable.

Close field iris diaphragm (13) until you can see the edges.

Focus the image of the field iris diaphragm by raising or lowering the condenser

using the condenser height adjustment knob (14).

Check if the circle of light is centered in the field of view. If not, use the two

condenser centering screws (15) to move the field iris diaphragm image to the center

of the field of view.

Open the field iris diaphragm until its image circumscribes the field of view.

Match the opening of the condenser aperture iris diaphragm (16) with the N.A. of the

objective in use in order to achieve the optimum objective performance.

NOTE: Most specimen are usually low in contrast, reducing the diaphragm opening

to 70%-80% of the N.A. value of the respective objective will generally provide an

image of acceptable quality.

To check the opening;

a) remove one ocular and look down the tube,

b) adjust the condenser iris so that 80% of the field is light,

c) replace the ocular,

a) set condenser aperture iris scale to about 80% of the N.A. value of objective,

b) example: N.A. of objective is 0.75, set the scale to 0.75 x 0.8 = 0.6.