Nikon A1+ User Manual - Page 6

Browse online or download pdf User Manual for Microscope Nikon A1+. Nikon A1+ 28 pages. Lbic nikon confocal
Also for Nikon A1+: Standard Operating Procedure (15 pages)

Print Page

Finding the sample by eye

1. Use the shortcut buttons (OC Panel) in Elements to select a wavelength to view.

The "Eyes" heading indicates the relevant buttons.

2. Set the microscope focus to course movement using the button the

right side of the microscope body.

3. It may not be obvious in which direction you need to move the lens to find the focal

plane. If you are unsure, lower the lens (nosepiece) as far as it will go using the

microscope focus knob (turn away from you to move the lens down). When it will

not lower any more (Z position on microscopes LCD display stays constant) start to

turn the focus knob towards you while looking down the eyepieces.

4. Use the OC Panel to switch filters & check your labelling at each wavelength

5. Press the "Fluorescence Off" OC button to turn off the fluorescence LED

6. You can check brightfield by eye using the Brightfield (Eyes) button

7. At this point you can either change to a higher magnification lens if required or

begin the scanning process

8. Lenses 60x and above always require a drop of oil between the lens and the

coverglass. 40X lenses can be either a dry or oil type. The touchpad used to select

the lenses tells you if the lens requires oil.