Nikon ECLIPSE 80i Gebruiksaanwijzing - Pagina 14

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ND filter sliders

Epi-illumination attachment

Brightness

1/4

1/8

1/16

1/32

1/64

1/128

1/512

Transmitted image in fluorescence observation

2.5

For fluorescence observations, turn off the microscope power switch to cancel the transmitted

image.

Bright ambient lights will make it more difficult to view the image. We recommend keeping the

room dark during fluorescence observations.

2.6

In Epi-fluorescence microscopy, image brightness can be adjusted by varying aperture

diaphragm size. Stopping down the aperture diaphragm darkens the image, while opening up

the aperture diaphragm makes the image brighter.

Use the aperture diaphragm of the Epi-fluorescence attachment or DIH-M after performing the

centering procedures. For a detailed discussion of this topic, refer to "17.5 Epi-fluorescence

Aperture Diaphragm."

Camera adjustment

2.7

(adjusting the brightness of the image on the monitor)

When observing images captured by the camera and displayed on the monitor, you can adjust

brightness by varying camera adjustment parameters, such as display mode, exposure mode,

metering mode, exposure compensation, and image level adjustment.

For detailed discussion, refer to the operating manual provided with the camera or camera

control software.

Individual Operations

2 Brightness Adjustment

ND4

ND8

ND filter sliders

DIH-M

ND16