Olympus Fluoview FV1000 Manual do utilizador - Página 4
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Olympus FV1000 User guide
August 3, 2011
D. Image Acquisition
Click off the light source button
to turn off
g
the non‐laser light.
d
Click on Dye list
button and double click
on the fluorescent dyes you want to use (e.g.
h
FITC and TRITC) from the list. Click Apply
b
c
button
(if Assign Dye Manually button
is
checked, uncheck before pressing Apply
button). It will activate the laser lines and set
f
detection channels up according to the dyes
you selected.
Choose Image size (default‐ 512x512) from
d
Size panel
.
Press
button (if not pressed) to
automatically adjust HV and Offset values
according to the scan speed change. Click
e
button to scan the sample. It will
scan fast at 2 us/pixel and show low‐quality
(pixelated) image in a Live View window.
c
b
While scanning, focus onto the region of interest with the remote focus controller or by clicking arrowhead
e
buttons in the microscope window (large arrowhead move the objective by the extent set by StepSize
,
and the small arrowheads moves the objective by the half of the large arrowhead step size).
Click Stop button
to stop scanning.
f
Set the Laser output level
(set the Multi‐Ar laser
to 1‐5% and the HeNe laser to 1‐25%, depending
on your sample condition, but no lower than 1%).
g
Set the scan speed
to 10~12.5 us/pixel and click
XY repeat
button to scan (you can scan
image faster or slower, depending on your sample).
Detector (CHS) Channel Setting
: Adjust the
brightness by HV (and Gain) and background black
level (Offset) of individual channels. Keep the HV
value below 700 (beyond 700, the background
noise will show up).