Olympus IX70 Manual de operação - Página 30

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If cells move during your experiment, you can drag one ROI on-the-fly to rack data while

collecting images. You can adjust all ROI at once (if you bump the dish slightly adding a drug) using the

RunExperiment Menu MOVE REGIONS. This jogs all ROI 1 or 10 pixel increments up to the point

where one of them hits the edge of the image. You can cheat and manually move the limiting ROI to then

allow all others to move again. If you save the images, you can analyze the data and step through frame-

by-frame to track objects, logging good data later on (this is always time consuming, however). There are

routines in ImageJ or MetaMorph to automatically track objects as an alternative.

The Scale16 and SetDisp buttons determine image appearance (not values). You can display fixed

ranges or autoscale each wavelength using Scale16. The Ratio appearances set using ranges in

ImageDispalyControls. MF doesn't have the ability to show true ratio numbers so it scales the colors

according to the range you specify (no floating point image type). You can insert a color bar for

calibrations of intensity (or calibration) values such as the one shown.

You need to close an experiment to analyze it, play it back as a movie (only possible if data were

saved). If you are doing another run, it is easier to use the GRAPHS menu item clear graphs and ROI,

then FILE, CloseMeasurementsFile, and CloseImageSave. This returns the software to the state where it

will ask you for a new name for ROI data and images. Remember to check the "save data" and "save

images" boxes in the ExperimentControlPanel (this is where it asks for names).

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