Olympus BH2 Series Manual - Page 2

Browse online or download pdf Manual for Microscope Olympus BH2 Series. Olympus BH2 Series 6 pages. Phase contrast
Also for Olympus BH2 Series: Troubleshooting & Adjustment Manual (46 pages), Complete Teardown, Cleaning, And Reassembly (23 pages), Complete Teardown, Cleaning, And Reassembly (40 pages), Teardown, Cleaning And Reassembly (45 pages), Installation Manual (9 pages)

Olympus BH2 Series Manual
Introduction ............................................................................................................................................................................ 3
Scope of this Document ...................................................................................................................................................... 3
What is Phase Contrast? ......................................................................................................................................................... 3
How Does Phase Contrast Work? ........................................................................................................................................... 3
Phase Contrast Types .............................................................................................................................................................. 3
Setting Up for Phase Contrast Microscopy ............................................................................................................................. 3
Match Selector Dial with the Low-Power Objective ........................................................................................................... 4
Replace Eyepiece with Phase-Centering Telescope ............................................................................................................ 4
Focus the Phase-Centering Telescope ................................................................................................................................ 4
Center the Selected Phase Annulus .................................................................................................................................... 5
Center the Remaining Phase Annuli ................................................................................................................................... 5
Replace Centering Telescope with the Eyepiece ................................................................................................................ 5
Place Green/Yellow Filter into Filter Receptacle ................................................................................................................ 5
Using Phase Contrast .............................................................................................................................................................. 6
Periodically Verify Centering of the Phase Annuli .............................................................................................................. 6
About the Phase Contrast Filter .......................................................................................................................................... 6
Phase Contrast Artifacts .......................................................................................................................................................... 6
How To Contact the Author .................................................................................................................................................... 6
Figure 1 - Set condenser dial to match the objective ............................................................................................................ 4
Figure 2 - Replace right eyepiece with phase telescope ........................................................................................................ 4
Figure 3 - Phase telescope improperly focused ..................................................................................................................... 4
Figure 4 - Adjust knurled focus ring to focus the image ........................................................................................................ 4
Figure 5 - The phase telescope is properly focused ............................................................................................................... 4
Figure 6 - Centering screws of BH2-PC/PCD condenser ......................................................................................................... 5
Figure 7 - The phase ring and phase annulus images ............................................................................................................ 5
Figure 8 - The phase annulus properly centered ................................................................................................................... 5
Figure 9 - Place green/yellow filter into filter receptacle ...................................................................................................... 6
Phase Contrast on the Olympus BH-2 Microscopes

Table of Contents

Table of Figures
Revision 1
Page 2 of 6