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Nikon N-STORM Manual de operação
Contents
Introduction ................................................................................................................................................ i
Chapter
1
Terminology Used in This Document........................................................................................... 1
Chapter
2
STORM Microscopy Operation ..................................................................................................... 4
2.1
Preparation of the N-STORM System ...................................................................................................... 5
2.2
Acquiring 3D-STORM (2D-STORM) Images.......................................................................................... 11
2.2.1
2.2.2
2.3
N-STORM Analysis ................................................................................................................................ 22
2.4
Terminating the N-STORM System ........................................................................................................ 28
2.5
Calibration for 3D-STORM ..................................................................................................................... 29
Chapter
3
The Screens of the N-STORM Software ..................................................................................... 34
3.1
N-STORM Control Window .................................................................................................................... 34
3.2
N-STORM Settings Window................................................................................................................... 36
3.3
N-STORM Analysis Window .................................................................................................................. 37
3.3.1
3.3.2
3.3.3
3.3.4
Acquiring Images in Normal Mode ........................................................................................... 11
Acquiring Images in Continuous Mode ..................................................................................... 16
Tool Bar .................................................................................................................................... 37
Slider ........................................................................................................................................ 40
Channel Tab ............................................................................................................................. 41
Status Bar................................................................................................................................. 42
Contents
ii